all bacteria are able to use citrate for energy or as a carbon source . that being said , only some bacteria can grow on citrate media . explain why bacteria that can use citrate for energy and carbon may not be able to grow on this media. (500 word ).
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Simmons citrate agar tests the ability of organisms to utilize citrate as a carbon source. ... If the medium turns blue, the organism is citrate positive. If there is no color change, the organism is citrate negative.Citrate agar is used to test an organism's ability to utilize citrate as a source of energy. ... that does not require the organism to use citrate as a sole carbon source. ... Thus citrate-negative bacteria can also grow on this medium. ... Some organisms are capable of growth on citrate and do not produce a color Citrate Test. Simmons citrate agar tests the ability of organisms to utilize citrate as a carbon source. Simmons citrate agar contains sodium citrate as the sole source of carbon, ammonium dihydrogen phosphate as the sole source of nitrogen, other nutrients, and the pH indicator bromthymol blue.Klebsiella pneumoniae and Proteus mirabilis are examples of citrate positive organisms. Escherichia coli and Shigella dysenteriae are citrate negative.The purpose is to see if the microbe can use the compound citrate as its sole source of carbon and energy for growth. ... Abundant growth on the slant and a change from green to blue in the medium indicates a positive test for growth using citrate.
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