Ammonia produced by photorespiration is refixed by
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Photorespiratory ammonia metabolism in isolated spinach (Spinacia oleracea L. cv. Viking II) mitochondria was measured using a selective ammonia electrode. The mitochondria showed high rates of ammonia production in the presence of glycine. The isolated mitochondria contained less than 0.02% of the glutamine synthetase activity present in the original homogenate and no significant reassimilation of the released ammonia could be observed with added glutamate or -ketogluterate. Exogenous added glutamine synthetase did reassimilate the released ammonia. In a recombinated system, with a chlorophyll to mitochondrial protein ratio equal to the ratio in vivo, chloroplasts could very effectively reassimilate the ammonia released in the mitochondria during oxidation of glycine.
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Photorespiratory ammonia metabolism in isolated spinach (Spinacia oleracea L. cv. Viking II) mitochondria was measured using a selective ammonia electrode. The mitochondria showed high rates of ammonia production in the presence of glycine. The isolated mitochondria contained less than 0.02% of the glutamine synthetase activity present in the original homogenate and no significant reassimilation of the released ammonia could be observed with added glutamate or -ketogluterate. Exogenous added glutamine synthetase did reassimilate the released ammonia. In a recombinated system, with a chlorophyll to mitochondrial protein ratio equal to the ratio in vivo, chloroplasts could very effectively reassimilate the ammonia released in the mitochondria during oxidation of glycine.
hope it will help U!
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