Question

Ammonium sulphate precipitation based on size of protein

Answer 1

Ammonium sulfate precipitation is one of the most commonly used methods for large and laboratory scale protein purification and fractionation that can be used to separate proteins by altering their solubility in the presence of a high salt concentration. ... This can be seen with the folding of recombinant proteins.

Answer 2

☛ Ammonium sulfate precipitation is one of the most commonly used methods for large and laboratory scale protein purification and fractionation that can be used to separate proteins by altering their solubility in the presence of a high salt concentration.

☛ Ammonium sulfate is an inorganic salt with a high solubility that disassociates into ammonium (NH4+) and sulfate (SO42−) in aqueous solutions.


☛ Ammonium sulfate is especially useful as a precipitant because it is highly soluble, stabilizes protein structure, has a relatively low density, is readily available, and is relatively inexpensive