Question

Amplification of DNA by PCR uses a DNA polymerase called:
(a) taqDNA polymerase
(c) DNA polymerase III
(b) RNA polymerase
(d) Reverse transcriptase​

Answer 1

Explanation:

500 U; 1 U/µl

Taq DNA Polymerase is a highly thermostable recombinant DNA polymerase of the thermophilic bacterium Thermus aquaticus. The enzyme catalyzes 5′→3′ synthesis of DNA, has no detectable 3′→5′ exonuclease (proofreading) activity and possesses 5′→3′ exonuclease activity. In addition, Taq DNA Polymerase exhibits deoxynucleotidyl transferase activity, which frequently results in the addition of extra adenines at the 3′-end of PCR products.

Recombinant Taq DNA Polymerase is ideal for standard PCR of amplicons 5 kb or shorter.

Supplied with 10X Taq Buffer with KCl; 10X Taq Buffer with (NH4)2SO4; 25 mM MgCl2.

Answer 2

Explanation:

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