Question

an enzyme has a km of 54 M in the absence of competitive inhibitior , and k apparent km of 10um in the presence of 34 M of inhibitor , calculate ki .
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Answer 1

Answer:

This is usually expressed as the Km (Michaelis constant) of the enzyme, an inverse measure of affinity. For practical purposes, Km is the concentration of substrate which permits the enzyme to achieve half Vmax.

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plotting v against v / [S] gives a straight line:

y intercept = Vmax.

gradient = -Km.

x intercept = Vmax / Km.

Answer 2

Answer:

Y intercept=V Max

X intercept=V Max/Km

Explanation:

Y intercept=V Max

X intercept=V Max/Km