Question

An enzyme is immobilized on solid surface. Assume that the external mass-transfer resistance for substrate is not negligible and that the michaelis-menten equation describes the intrinsic kinetics of enzyme reaction. A. Derive an expression which shows the relationship between the substrate concentration at the surface and that in the bulk solution.

Answer 1

The restriction of enzyme mobility in a fixed space is known as enzyme immobilization. Immobilization of enzymes provides important advantages, such as enzyme reutilization and elimination of enzyme recovery and purification processes and may provide a better environment for enzyme activity. Because enzymes are expensive, catalyst reuse is critical for many processes. Since some of the intracellular enzymes are membrane bound, immobilized enzymes provide a model system to mimic and understand the action of some membrane-bound intracellular enzymes. Product purity is usually improved, and effluent-handling problems are minimized by immobilization.

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