An enzyme preparation has activity of 2 units per 20 ul and protein concentration 0.4mg/ml. The specific activity of this enzyme will be
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Specific enzyme activity (SEA) = Enzyme activity (EA) / Enzyme conc. (EC)
In this case. EA = 2 U (2 umol/min) per 20 ul
Now, Talking about EC,
In 1 ml (or 1000 ul) amount of enzyme present is 0.4 mg (or 400 ug).
So, in 20 ul, amount of enzyme present is 400/1000 x 20 = 8 ug = 0.008 mg
So, SEA = 2 / 0.008 = 250 umol / min / mg
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