Question

an enzyme that follows Michaelis menten kinetics catalyzes the conversion of 35uM of substrate to product with a reaction velocity of 10uM per sec. The Km and Kcat for the substrate are 14uM and and 500 per sec respectively. The total amount of enzyme taken for the enzyme reaction is​

Answer 1

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• Km is defined as the concentration of substrate at which enzyme is working at half of maximum velocity. ... Explanation: At low substrate concentration, the rate of a reaction is determined by the rate of formation of an enzyme-substrate complex.