An established cell line can be called where it has been subcultured at least
A. 70 times at an interval of 3 days between subcultures
B. 40 times at an interval of 3 days between subcultures
C. 70 times at an interval of 1 day between subcultures
D. 50 times at an interval of 3 days between subcultures
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option (A) 70 times at an interval of 3 days between subcultures.
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