an experiment to show chlorophyll is necessary for photosyntesis step by step with:- aim, material required, procedure, observation, conclusion
Answers
Answer:
To prove that chlorophyll pigment in green leaves is necessary for the plants to prepare their carbohydrate (starch) food.
Requirements for the experiment:-
A well watered variegated leaf plant, beaker, water, Bunsen burner, mentholated spirit or rectified spirit or ethanol, iodine solution, a white porcelain tile.
Procedure for the experiments:-
A variegated leaf plant is taken for this particular experiment to prove the necessity of chlorophyll for photosynthesis. A variegated leaf is a leaf in which different patches of colors are present in a Croton or Coleus or Geranium plant. The place where green patches are present in the leaf, chlorophyll pigment is present while at the other color patches present in the leaf contain other pigments but not chlorophyll. Before starting the experiment to prove chlorophyll is necessary for photosynthesis an important step has to be carried out is known as De-starching. De-starching is a step in which the starch present in the leaves of the plant will be removed by keeping the variegated leaves plant in a dark room or closed cup-board for about 48 hours. This step is done to ensure that previously synthesized starch in the leaves get consumed up because photosynthesis doesn't occur at that time as the plant is kept in dark place.
After De-starching step the variegated leaf plant is kept in bright sunlight for about 6 hours. After this one of the variegated leaf is plucked out from the experimental plant. Then Iodine test is conducted for this experimental leaf to find out whether the leaf prepared starch through photosynthesis or not.
Explanation: