An insertion of an is element at random gene loci
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Insertion sequences (ISs) are small pieces of DNA which move within or between genomes using their own specialized recombination systems. They were discovered in the mid-1960s in studies of gene expression in Escherichia coli and its bacteriophages. Initially recognized by their ability to generate highly polar but unstable mutations in the gal and lac operons and in the early genes of bacteriophage lambda, they were later identified by electron microscopy as short insertions of DNA. The repeated isolation of a limited number of identical DNA sequences associated with these unstable mutations led to their being named ‘insertion sequences’.
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