Question

An oil immersion objective of a light microscope has a numerical aperture of 1.25. Using the Abbé
equation, the maximum theoretical resolving power (in nm) of the microscope with this objective
and blue light (wavelength = 450 nm) is ______

Answer 1

Numerical aperture may be termed as the light gathering ability of a lens. Numerical ... The maximum theoretical resolving power of a microscope with an oil immersion objective (NA = 1.25) and blue-green light is approximately 0.2 µ m . (0.5) 530 nm ... Approx. resolving power with light of 450 nm (blue light). 2.3 µ m.0