Question

Ans.4 Question 57: A 1 kb insert (1) is ligated to a 5 Kb vector (V) in a molar ratio of I:V of 5:1, in a reaction volume of 1 mL at a final DNA concentration at 10 ug/mL. How much of the insert and vector was used in the ligation mixture?
Answers : 1. 5 μg and 5 μg V
2. 2 ugland 8 ug V
3. 8 ug 1 and 2 ug V
4. 3 ug I and 7 ug V​

Answer 1

the insert and vector was used in the ligation mixture .

1. 5 μg and 5 μg V