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A. take a slide and clean it properly. drop 2-3drops of buttermilk on it and light a candle and then take the slide and just slightly get the heat from the flame by moving slide on it. Then cover it coverslip. Observe under microscope.
B.Two methods you can use first slide culture you put cover slip on Fungi colony then take it with care and safety consideration use of mask and bio safety cabinet put on glass slide put a drop methylene blue from slide margin you can watch directly Mycelium structure with its spore under microscope in 10 .
Second you must take a sample of colony fix it by alcohol and then staining it but may be you can watch a portion of Mycelium and spore in fixed condition but in mycology safety issues must be consider seriously because of opportunistic fungi risk.
C.by using hanging drop method.. i don't know about this method in breief sorry
D.If you are using a protozoa culture kit, protists will usually begin to appear after 24 hours with the most variety after about 3 days. Different creatures will grow at different depths of your cup of water, so take samples from different parts of the cup.
Use a pipet to take a sample of the water and place 1-2 drops on a plain microscope slide. Cover the drops with a coverslip.
Examine the slide with your microscope starting at 40x. Most protists have little color and are difficult to see in bright light, so turn your microscope diaphragm to the lowest light setting. It will take patience to adjust the lighting and focus the microscope.
Initially you will see very tiny dots moving around on the slide. Some move very rapidly, others more slowly. You can slow them down for observation by adding a drop of methyl cellulose, or you can place a few fibers from a cotton ball on the slide. The fibers will act as obstacles to prevent the protists from moving out of the field of view too quickly.
Once you find an area of protist activity on the slide, turn the magnification up to 100x or even 400x to see them better.
If no animals are visible, try again each following day. Many conditions, such as water hardness, temperature, and water acidity, can affect the growth and development rate of these organisms. Each succeeding day you will typically find more and different varieties of protozoa in your culture. Initially, smaller species will be prevalent. As the days pass larger species will appear. You will also see different algae forms appear. Certain species will be more common from the top of the cup and others from near the bottom. Gradually, food and water conditions will change, affecting the growth and development rates of the different protozoa.
5.Gram stain techinique is used
B.Two methods you can use first slide culture you put cover slip on Fungi colony then take it with care and safety consideration use of mask and bio safety cabinet put on glass slide put a drop methylene blue from slide margin you can watch directly Mycelium structure with its spore under microscope in 10 .
Second you must take a sample of colony fix it by alcohol and then staining it but may be you can watch a portion of Mycelium and spore in fixed condition but in mycology safety issues must be consider seriously because of opportunistic fungi risk.
C.by using hanging drop method.. i don't know about this method in breief sorry
D.If you are using a protozoa culture kit, protists will usually begin to appear after 24 hours with the most variety after about 3 days. Different creatures will grow at different depths of your cup of water, so take samples from different parts of the cup.
Use a pipet to take a sample of the water and place 1-2 drops on a plain microscope slide. Cover the drops with a coverslip.
Examine the slide with your microscope starting at 40x. Most protists have little color and are difficult to see in bright light, so turn your microscope diaphragm to the lowest light setting. It will take patience to adjust the lighting and focus the microscope.
Initially you will see very tiny dots moving around on the slide. Some move very rapidly, others more slowly. You can slow them down for observation by adding a drop of methyl cellulose, or you can place a few fibers from a cotton ball on the slide. The fibers will act as obstacles to prevent the protists from moving out of the field of view too quickly.
Once you find an area of protist activity on the slide, turn the magnification up to 100x or even 400x to see them better.
If no animals are visible, try again each following day. Many conditions, such as water hardness, temperature, and water acidity, can affect the growth and development rate of these organisms. Each succeeding day you will typically find more and different varieties of protozoa in your culture. Initially, smaller species will be prevalent. As the days pass larger species will appear. You will also see different algae forms appear. Certain species will be more common from the top of the cup and others from near the bottom. Gradually, food and water conditions will change, affecting the growth and development rates of the different protozoa.
5.Gram stain techinique is used
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