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Answers
Answer :
- Put some germinating seeds in a conical flask.
- The conical flask is fitted to a delivery tube bent twice at right angles by means of cork.
- Suspend a small test tube containing 20% potassium hydroxide.
- Dip the free end of the bent tube in a beaker of coloured water.
- Leave it for a day. Note the changes.
- The germinating seeds respires aerobically by utilizing the oxygen in the flask.
- Carbon dioxide released during the aerobic respiration is absorbed by potassium hydroxide and creates a vacuum and causes rise of water in the bent tube.
- If we put a burning candle in the setup, the candle extinguishes which proves oxygen is utilized by germinating seed for respiration.
Answer:
AIM
To show experimentally that carbon dioxide is given
out during respiration with the help of germinating
seeds.
MATERIALS REQUIRED
Conical flask, U-shaped delivery tube (tube bent twice
at right angles), cotton wool or moist blotting paper,
water, thread, beaker, test tube, rubber cork with one
hole, 20% freshly prepared KOH solution, vaseline,
soaked gram seeds.
THEORY
Respiration is a biochemical process during which food
(glucose) is oxidised to liberate energy. It is a catabolic
process.
In the experiment, moist gram seeds are taken as they
are actively respiring and releasing CO2. The CO2
released is absorbed by KOH and forms KgCO3.
PROCEDURE
1. Take about 25-30 seeds of a gram and germinate
these seeds by placing them on moist cotton wool or
moist blotting paper for 3-4 days.
2. Place the germinated seeds into a conical flask and
sprinkle a little water in a flask to moist the seeds.
3. Take freshly prepared 20% KOH solution in a test
tube and hang it in a conical flask with help of thread.
4. Close the mouth of the conical flask by placing a
rubber cork containing one hole.
5. Through the hole of rubber cork, insert one end of
the U-shaped glass delivery tube in the conical flask
and place the other end into a beaker filled with
water.
6. Seal all the connections of the experimental set-up
with vaseline so as to make it air-tight.
7. Mark the initial level of water in the U-shaped
delivery tube.
8. Keep the apparatus undisturbed for 1-2 hours and
note the change in the level of water in the delivery
tube.
OBSERVATIONS
After some time, the level of water in U-shaped delivery
tube dipped in the water of the beaker rises.
RESULT
Germinated gram seeds in a conical flask release CO,
during respiration. The C02 released is absorbed by
KOH is present in the hanging test tube in the conical flask.
This creates a vacuum in the conical flask which causes
upward movement of water in the delivery tube leading
to a change in the level of water in the delivery tube.
PRECAUTIONS
1. Germinating seeds should be kept moist.
2. All connections of the set-up should be air-tight.
3. Freshly prepared KOH solution should be used.
4. Keep one end of the U-shaped delivery tube in a conical
flask and the other end immersed in the water of the
beaker.
5. The test tube containing KOH should be hung
carefully.