Antibacterial agent in kupper cells in crypts of lieberkuhn
Answers
Answer:
Ask a colleague to split one of your cultures using your supplies and reagents. Ask a colleague for one of his/her cultures and split it using your colleague's supplies and reagents. Keep the two sets of cultures in the same incubator - preferably on the same shelf.
If the cultures that were handled by your colleague grow well, but the cells handled by you do not grow, the problem is YOU. One former member of my lab had this problem. It turned out to be caused by rough handling of single cell suspensions (pushing and pulling them through narrow gauge-needles with excessive force.) By the time the cells were aliquoted into new culture plates, most of them were dead. Therefore, if the first round of testing indicates that YOU are the problem, determine how many of the cells are still excluding trypan blue when you get ready to plate and how many are adhering to the surface of the culture flask after a reasonable period of time
Explanation:
Copper and its alloys (brasses, bronzes, cupronickel, copper-nickel-zinc, and others) are natural antimicrobial materials. Ancient civilizations exploited the antimicrobial properties of copper long before the concept of microbes became understood in the nineteenth century.[1][2] In addition to several copper medicinal preparations, it was also observed centuries ago that water contained in copper vessels or transported in copper conveyance systems was of better quality (i.e., no or little visible slime or biofouling formation) than water contained or transported in other materials.[citation needed]