Biology, asked by jains7470, 1 year ago

apotosis of human osteosarcoma mg-63 cells induced by dihydroartemisinin. chinese

Answers

Answered by rajavignesh103
0

The apoptosis effects of dihydroartemisinin (DHA) on human osteosarcoma MG-63 cells have been studied using the methods of CCK-8 staining, flow cytometry analysis, Hoechst33258 staining, Annexin VFITC staining and electron microscope. The results showed that DHA decreased the cell viability in a concentration and time-dependent manner. The IC50 values of DHA against the MG-63 cells were determined as 44.87 μmol/L and 19.15 μmol/L after 48 h and 72 h, respectively. The results of flow cytometry analysis showed that DHA could induce the emergence of the phase of apoptosis, the rate is 45.10% and the cell cycle is blocked in G0/G1 phase. Cell nucleus concentrated and appeared granular fluorescence by Hoechst33258 staining. This study suggested that DHA had induced apoptosis of the human osteosarcoma MG-63 cells effectively, and provided foundation and research proofs to study more about the apoptosis mechanisms of the osteosarcoma cells

Similar questions