Application of beer's law in quantitative analysis
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Beer’s law in quantitative analysis: Modern chemical analysis has routinely used spectrophotometry or quantitative use of beer’s law in agricultural, clinical, environmental, pharmaceutical, and quality control laboratories for over fifty years. Spectrophotometry is the study of absorption or emission of light by a chemical species. The versatility and ease of spectrophotometry makes it a cost-effective way to analyze large numbers of samples and even provide in-line quality assurance for the manufacturing of food, beverage, agrochemicals, and pharmaceuticals. For example, this technique is routinely used in the beverage industry to monitor phosphates, sugars, and coloring agents in soft drink
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Applications. Beer-Lamberts law is applied to the analysis of a mixture by spectrophotometry, without the need for extensive pre-processing of the sample. Examples include the determination of bilirubin in blood plasma samples. The spectrum of pure bilirubin is known thus the molar absorbance is known.
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