Application of complexes in anti ulcer
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2.2. Plant Material and Preparation of Extract
Spondias mombin leaves were collected in Cajazeirinhas Municipality, Paraíba, Brazil (S 6°58.34.558–W 37°48.21.424), in July 2014. A voucher specimen was deposited in the Herbarium of the Agronomic Institute (IPA) on the registration number 89987. The leaves (2000 g) were air dried (oven with forced air circulation at 40°C).
Thereafter, the plant material (2000 g) was subjected to cold exhaustive maceration with three separate solvents (2000 mL) of different polarities (hexane, ethyl acetate, and ethanol) under agitation for three consecutive periods of 72 h intervals each. The extracts were filtered, and the solvents were completely removed with the aid of a low-pressure rotary evaporator. The ethanolic extract presented a satisfactory yield (7.3% w/v), which was superior to the other extracts.
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