Applications of differential interference contrast microscopes
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=>Light source emits unpolarized light into microscope and is immediately oriented to 45° by the first polarizing filter.
=>Polarized light enters Wollaston prism and is separated into two rays at 90° to one another.
=>Both rays are focused by condenser lens to sample at specific points.
=>Rays propagate through sample at a separation point which mimics the resolution of the microscope (0.5μm resolution, rays focused at 0.5μm separation). Light is collected by objective lens and focused to second Wollaston prism.
=>Second Wollaston prism combines two rays into one polarized ray at 135°, which results in improved contrast in the sample image: improvements in bright spots, dark spots, and overall focus.
=>Light source emits unpolarized light into microscope and is immediately oriented to 45° by the first polarizing filter.
=>Polarized light enters Wollaston prism and is separated into two rays at 90° to one another.
=>Both rays are focused by condenser lens to sample at specific points.
=>Rays propagate through sample at a separation point which mimics the resolution of the microscope (0.5μm resolution, rays focused at 0.5μm separation). Light is collected by objective lens and focused to second Wollaston prism.
=>Second Wollaston prism combines two rays into one polarized ray at 135°, which results in improved contrast in the sample image: improvements in bright spots, dark spots, and overall focus.
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