Question

Applications of thin layer chromatography?

Answer 1

Purity of any sample: Purity of sample can be carried out with TLC. Direct comparison is done between the sample and the standard or authentic sample; if any impurity is detected, then it shows extra spots and this can be detected easily.

Identification of compounds: Thin layer chromatography can be employed in purification, isolation and identification of natural products like volatile oil or essential oil, fixed oil, waxes, alkaloids, glycosides, steroids etc.

Examination of reactions: Reaction mixture can be examined by Thin layer chromatography to access whether the reaction is complete or not. This method is also used in checking other separational processes and purification processes like distillation, molecular distillation etc.