Question

Arrange the events in the order that they occur during DNA replication. Helicase unzips DNA to separate strands. Polymerase synthesizes new DNA strands. Topoisomerase unwinds the coiled DNA. The new strands are glued using ligase. Primase lays down short RNA strands

Answer 1

Answer:

The Invitrogen Anza™ T4 DNA Ligase Master Mix facilitates the joining of abutted 5’-phosphate and 3’-hydroxyl termini in duplex DNA through the formation of a phosphodiester bond. It can be used to join DNA fragments with both sticky ends and blunt ends, and to repair nicks in double-stranded DNA with 3'-hydroxyl and 5'-phosphate ends. Anza T4 DNA Ligase is formulated as a 4X concentrated master mix. Ligation can be performed with DNA in water, TE, elution buffer, or 1X Anza™ buffers.

Benefits:

• Ligation complete in 15 minutes at room temperature

• A single ligase master mix for both sticky-end and blunt-end ligation reactions

• Ready-to-use master mix format reduces pipetting steps

• 4X concentration enables more dilute DNA to be used in ligation reaction without concentrating

Anza T4 DNA Ligase Master Mix is part of the Anza™ Restriction Enzyme Cloning System, unifying the traditional cloning processes. Other Anza DNA Modifying Enzymes include the Anza T4 PNK Kit, Anza DNA Blunt End Kit, and Anza DNA End Repair Kit.