Question

Arrange the following steps of DNA fingerprint
in sequence -
i. Hybridization using labelled VNTR probe
ii. Isolation of DNA
iii. Detection of hybridized DNA Fragment by
Autoradiography
iv. Southern Blotting
v. DNA Fragment separation by Gel
Electrophoresis
vi. Digestion of DNA by Restriction Endonuclease
vii. Alkali Treatement to Denature DNA​

Answer 1

Answer:

Did you mean: Arrange the following steps of DNA fingerprint in sequence - i. Hybridization using labelled VNTR probe ii. Isolation of DNA iii. Detection of hybridized DNA Fragment by Autoradiography iv. Southern Blotting v. DNA Fragment separation by Gel Electrophoresis vi. Digestion of DNA by Restriction Endonuclease vii. Alkali Treatment to Denature DNA

Explanation:

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Answer 2

Steps of DNA Fingerprint (Southern Blotting techniques):

1. DNA extract from cell.
2. Cutting DNA by restrictions Endonuclease.
3. Separate DNA fragments on Electrophoresis gel.
4. Transfer DNA onto paper.
5. Adding the radioactive probe.
6. Setting up the X-ray film.