Arrange the various steps of DNA fingerprinting technique in the correct order,
i.Separation of DNA fragments by electrophoresis
ii.Digestion of DNA by restriction endonucleases
iii.Hybridization using VNTR probe
iv.Isolation of DNA
v.Detection of hybridized DNA fragments by autoradiographing.
vi.Transferring separated DNA fragments to nitrocellulose membrane
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1) Isolation of DNA: The genetic material is contained inside the cells. It has to be obtained in pure form without even the attached histones and other proteins.
2) Digestion of DNA by restriction endonuclease: The DNA fragments with the desired genes are cut to the specific ends.
3) Separation of DNA fragments by gel electrophoresis.
4) Hybridisation using VNTR probe
5) Detection of hybridized DNA fragments by autoradiographing.
6)Transferring separated DNA fragments to nitrocellulose membrane.
2) Digestion of DNA by restriction endonuclease: The DNA fragments with the desired genes are cut to the specific ends.
3) Separation of DNA fragments by gel electrophoresis.
4) Hybridisation using VNTR probe
5) Detection of hybridized DNA fragments by autoradiographing.
6)Transferring separated DNA fragments to nitrocellulose membrane.
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