Question

Assertion (A) : Chromatography is used to separate pigments from natural colours like chlorophyll.

Reason (R) : Coloured components of a mixture can be separated by using an adsorbent on which they

are adsorbed at different rates.​

Answer 1

Answer:

A and R are correct and is the correct explanation of A

Answer 2

Complete question:

Although a part of this question is missing, we might be referring to the following question:

Mark the correct choice as:

(A) If both assertion and reason are true and the reason is the correct explanation of the assertion

(B) If both assertion and reason are true but the reason is not the correct explanation of the assertion.

(C) If the assertion is true but the reason is false

(D) If both assertion and reason are false.

Answer:

(A) If both Assertion(A) and Reason(R) are true and the Reason is the correct explanation of the Assertion(A).        

Explanation:

• The word 'chromatography', composed of the Greek term 'Khroma' means colour and 'graphein' meaning to draw a graph, was coined by the Russian botanist M.S.Tswett (1906), to describe the process of separating mixtures of plant pigments such as chlorophylls, β-carotene, xanthophylls etc.
• Chromatography is a simple laboratory technique that is widely used to separate, purify and identify compounds in a mixture of components.
• It is a physical process, where components of a mixture are separated as a result of their differential distribution (i.e. the partition of solute) between a mobile phase (moving) and a stationary phase.
• The more its movement shows the interaction of the substance with the stationary phase.  
• The general principle employed is the movement of a mixture of compounds over a mobile phase, which passes over a selectively adsorbing surface using an adsorbent, the stationary phase.

• Some compounds adsorb more strongly to the stationary phase than others, as they elute the column at a very slow rate.  Thus they can be separated on the basis of their elution rate accordingly.
• For example, The dark colour of the spinach is a combination of β-carotene and chlorophylls.  
• Therefore, both assertion and reason are true and the reason is the correct explanation of the assertion, i.e. option A) is correct.

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