Assertion : Hybridoma cells are shifted to a medium deficient in nutrient which cannot be synthesized by myeloma cells.
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The hybridoma cells are shifted to a medium deficient in nutrient which cannot be synthesized by, myeloma cells so that myleoma cells could be removed or hybridoma cells could be purified. The unfused myleoma cells die while hybridoma cells survive. The hybridoma cells are allowed to multiply separately.
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