Question

Assumptions of michaelis menten equation

Answer 1

Three assumptions are implicit in Michaelis-Menten kinetics: the steady-state approximation, the free ligand approximationand the rapid equilibrium approximation. (TheBriggs-Haldane approach frees us from the last of these three.) Now consider the second assumption in the list: the free ligand approximation.

Answer 2

This equation is called the Michaelis–Menten equation. Here,  represents the maximum rate achieved by the system, at saturating substrate concentration. The Michaelis constant  is the substrate concentration at which the reaction rate is half of .[1] Biochemical reactions involving a single substrate are often assumed to follow Michaelis–Menten kinetics, without regard to the model's underlying assumptions.