Question

At what temperature the DNA molecule of interest is denatured ?

Answer 1

in PCR 94degree celcius

Answer 2

Answer:

The DNA molecule of interest or which has been taken to amplify is denatured at 94°C.

In Polymerase Chain reaction, The DNA molecule is separated or denatured to make it single stranded.

This phase is known as Initiation phase and strands are denatured so that they can be synthesized with the desired primers to diagnose some particular disease related to a gene.

The other two steps of Polymerase chain reaction are elongation and termination.