At what temperature the DNA molecule of interest is denatured ?
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The first temperature in PCR is about 90°C. At this high temperature, the template DNA is melted or denatured. The hydrogen bonds between the nitrogenous bases are broken and the double-stranded helix becomes two single strands of DNA.
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Heating. Theoretically the 86-bp DNA fragment will be completely denatured during the heating process at 95°C since the melting temperature (Tm) of the DNA was calculated to be 76.2°C according to Wallace et al.
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