Attenuating effects of ascorbic acid on cypermethrin induced histological and biochemical changes in developing brain of gallus domesticus
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Cypermethrin [cyano-(3-phenoxyphenyl) methyl] 3-(2,2-dichloroethenyl)-2,2-dimethylcyclopropane-1-carboxylate), second generation, type-II, pyrethroid insecticide having cyano group which has broad spectrum uses in agricultural sector. Present study aimed to investigate biochemical and histological changes induced by cypermethrin and protective effect of vitamin C in embryonic brain of developing chick of Gallus domestius. Fertilized eggs were divided into four groups having 30 eggs each. Group A: served as control, Group B: as vehicle which was administered with distilled water by immersion method, Group C: treatment group; was treated with 25 mg/ml of cypermethrin via immersion method and Group D: recovery group; supplemented with 25 mg/ml of cypermethrin along with 100 mg of vitamin C by air sac method. At embryonic day 0 (ED 0), all the groups treated with requisite doses for 60 minutes. Eggs were recovered and cerebellum was excised and further processed for biochemical and histological changes on embryonic day 16 (ED 16).