Question

b. You repeat the experiment fixing your mistakes and are excited when you see red dots
around the nucleus (stained by DAPI). You show it to a senior colleague in the lab who isn't
convinced with the result. You show him the data from the vendor which proves that the
primary antibody works You tell your colleague that you used Indirect immunofluorescence
microscopy with a secondary antibody tagged to RFP (red fluorescence proteinl. Your colleague
brushes off your result by saying the red dots you see are from non-specific binding (bound to
something other than the intended target of the secondary antibody. How would you convince
your colleague that the red doty you see on the imare do Indeed show your transfection
worked (As we have not covered other techniques such as westem blotting,
assume you are
not allowed the other tecniques to prove your result) (3 polnts)​

Answer 1

Answer:

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Explanation: