Question

Bacterial transformation method of gene cloning hiology discussion

Answer 1

A straight-forward way of doing this is to identify the gene in a clone by its product. For example, if the plasmid contains a gene conferring resistance to say ampicillin, only those transformed bacteria containing the plasmid can grow in an ampicillin-containing agar and the non-transformed bacteria are eliminated.

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Answer 2

Answer:

Hey mate answer of your question is given below by me..

Explanation:

Isolation of DNA [gene of interest] fragments to be cloned. Insertion of isolated DNA into a suitable vector to form recombinant DNA. ... Selection of transformed host cells and identification of the clone containing the gene of interest. Multiplication/Expression of the introduced Gene in the host.

I hope it can help you..