Basic composition and role of water of culture media
Answers
Answer:
CULTURE MEDIA:
Culture media gives artificial environment simulating natural conditions necessary for growth of bacteria. The basic requirements for culture media are:
Energy source.
Carbon source.
Nitrogen source.
Salts
Optimum pH.
Adequate oxidation-reduction potential.
Growth factors.
Components of culture media:
Water– Source of hydrogen and oxygen.
Electrolytes– NaCl & other electrolytes.
Peptone– Mixture of partially digested proteins (animal or vegetable). Commercial peptone.
Meat extract, yeast extract – Protein degradation products/carbohydrates/Inorganic salts/Growth factors.
Blood– It enriches media. 5-10 % defibrinated horse or sheep blood (serum).
Agar– Sea-weed (Algae-geledium species) Carbohydrates / Protein-like material / Fatty acids. 2-3 % concentration. It melts at 950 C and solidifies at 420 Agar acts merely as solidifying agent of culture medium and does not provide any nutrition to the bacteria.
Classification of media:
Media have been classified in many ways:
Solid / Liquid / Semisolid
Simple / Complex / Synthetic / Special
Aerobic / Anaerobic