Beginning with 600 template dna molecules after 25 cycle of pcr how many amplification will be produced
Answers
Polymerase chain reaction, better known as PCR, is one of the technologies that not only made a tremendous impact on the scientific community, but also affected many aspects of our everyday lives. More than 30 years ago, the introduction of recombinant DNA technology as a tool for the biological sciences revolutionized the study of life. Molecular cloning allowed the study of individual genes of living organisms; however this technique was dependent on obtaining a relatively large quantity of pure DNA. This depended on the replication of the DNA of plasmids or other vectors during cell division of microorganisms. Researchers found it extremely laborious and difficult to obtain a specific DNA in quantity from the mass of genes present in a biological sample. A technique that amplifies DNA through a simple enzymatic reaction, was developed by Karry Mullis at that time which enabled scientists to make millions - or even billions - of copies of a DNA molecule in a very short time.