Question

Bioconversion glucose to ascorbic acid

Answer 1

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l-Ascorbic acid is an important product currently made using the Reichstein process, which is mainly chemical. Recently, bacteria have been identified that are able to transform in a very efficient way glucose to 2,5-keto-d-gluconic acid and this product to 2-keto-l-idonic acid, precursor ofl-ascorbic acid. When the corresponding strains are used toegther, it is possible to get 2-keto-l-idonic acid directly from glucose. Moreover, new strains have been constructed by introducing a gene from a strain responsible for the second step into a strain responsible

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