Question

blood smear is stained with which stain option are
methylene blue
saffranin
leishman stain
carbol fuchsin

Answer 1

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Blood smear is stained with Leishman Stain.

$\huge{\bold{\underline{Additional \: Points:-}}}$

• It is Used to stain blood Cells.

• It is used to differentiate the White blood cells & Red blood cells from the Parasites of disease causing Like Malaria & Trypanosoma.

• It has a Composition of :- Methylene Blue & Eosin.

• It was invented by William Boog Leishman.

• Commonly Leishman stain is sold in Powder Form.

• It should be Stored in Dark conditions as Light Oxidizes the Stain (Leishman stain)

• On Staining The blood cells (WBC & RBC) it gives a characteristic Violet colour which helps in differentiation of the Blood cells From Pathogens.

• In some areas of Biology the stain (Leishman stain) is used to stain the Chromosomes of Some organisms.

• The pH of Leishman stain is Neutral I.e pH = 7.

Answer 2

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Leishman Stain