Brief note on transmission electron microscopy
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Transmission electron microscopy [ TEM ] is a microscopy technique whereby a beam of electrons is transmitted through an ultra thin specimen, interacting with the specimen as it passes through.
An image is formed from the interaction of the electrons transmitted through the specimen;
the image is magnified and focused onto an imaging device, such as a fluorescent screen, on a layer of photographic film, or to be detected by a sensor such as a CCD camera.
Let's discuss 'What are its Advantages n' Disadvantages.. ?'
¡¡ DISADVANTAGES ¡¡
♦ Low sampling volume and rather slow process of obtaining information.
♦ High capital and running cost.
♦ Special training required for the operation of the equipment.
♦ Difficult sample preparation. Possibility of electron beam damage.
♦ Samples which are not stable in vacuum are difficult to study.
♦ Magnetic samples require special care.
♦ Non-conducting samples require gold or carbon coating.
♦ Difficulty in the interpretation of images. In usual mode of operation information is integrated along the beam direction.
¡¡ ADVANTAGES ¡¡
♦ Real (Image) and reciprocal space (diffraction pattern) information can be obtained from same region of sample.
♦ Chemical information via EDX and EELS possible (with additional attachments).
Energy filtered images possible via EELS filter.
♦ High resolution imaging possible (via HRLFI & HAADF in STEM).
♦ Possible to obtain amplitude and phase contrast images. Many different kinds of phase contrast images can be obtained.
Hope it helps! :D
Transmission electron microscopy [ TEM ] is a microscopy technique whereby a beam of electrons is transmitted through an ultra thin specimen, interacting with the specimen as it passes through.
An image is formed from the interaction of the electrons transmitted through the specimen;
the image is magnified and focused onto an imaging device, such as a fluorescent screen, on a layer of photographic film, or to be detected by a sensor such as a CCD camera.
Let's discuss 'What are its Advantages n' Disadvantages.. ?'
¡¡ DISADVANTAGES ¡¡
♦ Low sampling volume and rather slow process of obtaining information.
♦ High capital and running cost.
♦ Special training required for the operation of the equipment.
♦ Difficult sample preparation. Possibility of electron beam damage.
♦ Samples which are not stable in vacuum are difficult to study.
♦ Magnetic samples require special care.
♦ Non-conducting samples require gold or carbon coating.
♦ Difficulty in the interpretation of images. In usual mode of operation information is integrated along the beam direction.
¡¡ ADVANTAGES ¡¡
♦ Real (Image) and reciprocal space (diffraction pattern) information can be obtained from same region of sample.
♦ Chemical information via EDX and EELS possible (with additional attachments).
Energy filtered images possible via EELS filter.
♦ High resolution imaging possible (via HRLFI & HAADF in STEM).
♦ Possible to obtain amplitude and phase contrast images. Many different kinds of phase contrast images can be obtained.
Hope it helps! :D
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