Question

Buffering capacity depends on absolute concentration of cell and acid

Answer 1

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The $\bold{buffering\:ability}$ is highest when the conjugate base [A-] and weak acid[HA] are present in equal concentrations (d = 0.5). If d is much higher or lower than 0.5, then additional acid or base will result in a more dramatic change in pH. This dissociation has a pKa

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Answer 2

Rhodamine 123 as a probe of transmembrane potential in isolated rat liver mitochondria