Question

By reacting peptide with anhydrous hydrazine which
terminal is isolated as free amino acid?बाय रिएक्टिंग विद अनहाइड्रेस हाइड्रेस इन विच टर्मिनल इन आइसोलेटेड एचपी अमीनो
एसिड ​

Answer 1

Answer:

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Explanation:

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Answer 2

By reacting peptide with anhydrous hydrazine, the Carboxy terminal is isolated as free amino acid.

• Anhydrous hydrazine is used to break down proteins and peptides, and the free amino acids that are liberated from their carboxyl termini are then examined as phenylthiocarbamyl (PTC) derivatives by reverse-phase chromatography.
• The most used chemical technique for identifying C-terminal amino acids, as well as occasionally their nearby residue, is hydrazinolysis.
• Hydrazinolysis is the process of releasing amino acids once hydrazine breaks the peptide bonds in a protein.
• Potential sources of error in the procedures used to identify end groups in macromolecules are numerous enough with hydrazinolysis to warrant skepticism about any conclusion until it is supported by additional techniques.
• The best method for assessing the outcomes of hydrazinolysis is to use carboxypeptidases.
• Proteins' main structures affect how easily they can be hydrazinolyzed, hence the best conditions must be found for each individual protein being studied.
• This is accomplished by experimenting with different reaction parameters, such as duration, temperature, catalyst usage, and stringent water exclusion.

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