Bypass reactions in reversal of glycolysis
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The three reactions of glycolysis that proceed with a large negative free energy change are bypassed during gluconeogenesis by using different enzymes. These three are the pyruvate kinase, phosphofructokinase-1 (PFK-1) and hexokinase/glucokinase catalyzed reactions.
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Gluconeogenesis uses many of the same reactions as glycolysis, but running in reverse. Among theglycolytic reactions, recall that all are readily reversed with the exception of the reactions catalyzed by hexokinase/glucokinase, phosphofructokinase, and pyruvate kinase. ... In gluconeogenesis, they arebypassed.
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