c) Write down the steps of preparing a slide to observe a cheek cell?
Answers
1) Take a clean cotton swab and gently scrape the inside of your mouth.
2) Smear the cotton swab on the centre of the microscope slide for 2 to 3 seconds.
3) Add a drop of methylene blue solution and place a coverslip on top. Concentrated methylene blue is toxic if ingested. Wear gloves and do NOT allow children to handle methylene blue solution or have access to the bottle of solution.
4) Remove any excess solution by allowing a paper towel to touch one side of the coverslip.
5) Place the slide on the microscope, with 4 x or 10 x objective in position and find a cell. Then view at higher magnification.
Answer:
Explanation:
1. Rinse your mouth to remove any food particles.
2. With the help of the toothpick scrap the inner side of the cheek to get some epithelial cells.
3. Place the scrappings in a drop of water over a clean slide.
4. Spread the scrappings with the help of needle.
5. Add a drop of methylene blue over the slide.
6. Wait for 2 minutes and then remove the extra stain with blotting paper.
7. Add a drop of glycerine on the slide.
8. With the help of needle, gently place the coverslip over the slide to avoid entry of air bubbles.
Observe the slide under the microscope.