calculate glucose content of urine when volume of urine sample required for 10 ml Benedict's quantitative reagent is 12 ml and dilution factor is 5
Answers
Answer:
10ml Benedict's reagent is 12ml and dilution factor is 5. 2.
Answer:
0.5% is the approximate glucose content in the urine sample.
Explanation:
Benedict's test
It is a quick laboratory procedure used to find out whether glucose is present and how much of it is in a liquid sample. Given that it is a qualitative test, you can only get an estimate of the sample's glucose concentration from it. Benedict's test is used to find out whether glucose is present in bodily fluids like blood, urine, or stool.
Using the given data, you would need to perform a series of calculations using Benedict's test method to determine the urine's glucose content. Benedict's reagent, which has copper ions and reacts with glucose to create a coloured complex, is used in this procedure. The amount of glucose present in the sample directly relates to how intense the colour is.
Finding the original glucose concentration in the urine sample before it was diluted is the first step. To do this, you would calculate the original concentration using a dilution factor of 5.
Original glucose concentration = Dilution factor / Concentration of Benedict's Reagent taken
∴Glucose Concentration = 5 / 10 = 0.5
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