Question

calculate the molecular mass of Penicillium ​

Answer 1

Explanation:

Penicillium chrysogenum produces not only penicillin but also a low molecular mass (6.5 kD) protein (PAF, GenBank accession number AAA92718).

Answer 2

Answer:

Purification of PAF

Purification of PAF was carried out as described previously.21, 23 Briefly, P. chrysogenum Q176 was grown in a sucrose (20 g/L) and NaNO3 (3 g/L) based minimal medium for 96 h at 25 °C with shaking. After harvesting of the mycelia by centrifugation and separation of the low molecular weight protein fraction in Amicon Stirred Cells (V=50 mL, Biomax PBTK ultrafiltration disks, size exclusion limit MWCO=30 000; MILLIPORE, Billerica, MA, USA), PAF was purified by ion-exchange chromatography on a CM Sephadex Fast Flow column (2 × 18 cm, equilibrated with 50 mm sodium phosphate buffer, pH=6.6, flow rate 1 mL/min, t=4 °C; AMERSHAM-Pharmacia, Uppsala, Sweden). PAF was eluted with a NaCl gradient (0.05–1 m) prepared in the equilibrating buffer, and PAF-containing fractions were dialyzed in phosphate-buffered saline (PBS).

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