Question

Can anyone provide
practical t. S of dicot root and monocot root with the help of material and procedure? ​

Answer 1

Answer:

Aim:-

To prepare a temporary stained mount of a transverse section of dicot and monocot stem and root to study various plant tissues.

Material Required:-

Preserved material of sunflower root and stem

Preserved or fresh material of maize root and stem

Microscope

Sharp blade

Slides

Watch glass

Coverslips

Safranin (1gm in 100ml of 50% ethanol)

Brush

Glycerine

Blotting paper

Procedure:-

Taking Sections

Hold the dissected plant material between your index finger and thumb, while keeping the edge of the razor perpendicular to the longitudinal axis of the plant. Slice it into thin sections.

Using the edge of the blade, shift these sections into a watch glass with the help of a brush. The watch glass must hold water.

Process Of Staining

Pick 2 to 4 thin and good transverse sections. Shift it to a different watch glass holding safranin stain.

Let the complete set rest in the stain for a couple of minutes.

After a while, drain the sections of the stain and rinse again with water so as to wash off the excess strain.

Mounting

On a clean slide, place a stained section in the middle of the slide, mounting water or glycerine.

Using a needle slowly places the coverslip.

An excess of water or glycerine from the edge of the coverslip can be removed by placing blotting paper.

Ensure, that the air bubbles are not formed while mounting.

Precautionary Measures

While dissecting the section, both the blade and the material should be supplied with adequate water.

While working with sections, use a brush.

Gently place the coverslip in order to avoid air bubbles.

Excess glycerine can be removed with filter paper