Question

Can DNA be amplify in vitro also ?

Answer 1

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molecules could only be amplified in vivo in appropriate host cells. Here, we describe an in vitroprocedure, ligation-during-amplification (LDA), for selectiveamplification of closed circular DNAusing sequence-specific primers. ... Through thermal cycling, closed circular DNA is amplifiedexponentially.


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Answer 2

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The polymerase chain reaction is a powerful technique used to amplify nucleic acids in vitro. ... Here, we describe and demonstrate an in vitro procedure, ligation-during-amplification (LDA), for selective amplification of closed circular DNA using sequence-specific primers.