Can i have a procedure or proctocol that i can use to extract total proteins from egg whites
Answers
Answered by
0
Answer:
Separate the yolk from the white by cracking the egg and keeping the yolk inside. Be sure to collect the white in a suitable container/beaker.
Add about 100mL distilled or deionized water to the egg white and stir until a white precipitate forms. These are the egg globulins.
Now you can either centrifuge the mixture and pour off and save the supernatant which is your ovalbumin. Or you can simply filter the mixture and keep the filtrate. I'd recommend the centrifugation as it's neater and a cleaner separation.
Feel free to further separate the albumin via ammonium sulphate precipitation from it's solution.
Explanation:
Similar questions