Question

Can over heated agar be used for plating?

Answer 1

Answer:

Only as much agar as is needed for the experiment should be melted as it cannot be re-used. Ten minutes before pouring plates, the tubes of melted agar should be transferred from the 55 °C water bath to a heat block on the laboratory bench set at 48 °C. ... If the agar is too hot, the bacteria in the sample may be killed.

Explanation:

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