Question

Can paecilomyces lilacinus be mass multiplied in jaggary water solution

Answer 1

Answer:

Paecilomyces lilacinus was mass multiplied in both solid substrates and liquid media under in vitro condition. Among solid substrates tried, sorghum grains encouraged maximum spore production (327.78 × 106 cfu /g at 20 Days After Inoculation) followed by coconut oil cake. When various duration of incubation were tried, maximum spore production was observed at 20 DAI (Days After Inoculation) followed by 30 DAI. Optimum duration to get maximum spore load from sorghum grains was 21.3 days. From the liquid media tried, coconut water collected from matured nuts (West Coast Tall) supported maximum dry mycelial weight. Vermiwash at 1: 10, 1:20,1:40,1:60 and 1:100 did not support the growth of the fungi. When coconut water collected from different stages of maturity viz., 6-8, 8-10 and 10-12 months was tested, 10-12 months recorded maximum dry mycelial weight. Optimum duration to get maximum dry mycelial weight was 7 DAI followed by 14 DAI in coconut water. Effect of temperature on the growth of P. lilacinus was studied in solid substrate (sorghum grain) and liquid media (coconut water) at different temperatures viz., 15,20,25,30 and 35+1°C. Number of colony forming units in sorghum grains was found to be maximum at 30+1°C followed by 35+1°C. In liquid media (coconut water) also maximum dry mycelial weight was recorded at 30+1°C which was on par with 35 and 25+1°C. Effect of temperature on the mycelial growth of P