Question

Characteristics features of type 2 restriction enzymes

Answer 1

Type II Restriction Enzymes

• The type 2 restriction endonucleases mostly recognise palindromic sequences to cut the DNA.

• The palindromic sequence consists of 4 – 6 basepairs.

• It is bilaterally symmertrical.

• The base sequence in one strand is same in the other strand while reading in reverse order.

• An axis or line cuts the palindromic sequence in to two identical halves, and is called axis of symmertry.

• Some restriction enzymes cut at the resrtiction site along the axis of symmetry.

• Others cut it at either side of the axis of symmetry.