chardakov method practical diagram for notes
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The Chardokov method provides a quick means to determine plant tissue water potentials. This method depends on the change in density in a solution that occurs after a tissue has been immersed in it. The solution gains or looses water depending on the water potential of the tissue. If the density of a solution does not change (no net movement of water) then this solution has the same water potential as the tissues that were incubated in it. It is assumed that solute movement between tissue and solution is negligible. Density changes can be observed by watching whether a drop of the original solution floats or sinks in the test solution after tissue incubation. Alternately, for a more accurate measurement of changes in the solution density, a refractometer can be used.